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His-HRP Mouse Monoclonal Antibody

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells.

Product Name: His-Tag Mouse Monoclonal Antibody-HRP conjugated

Isotype: IgG1

Clone Number: 6E8

Storage Buffer : PBS, pH 7.4, 50% Glycerol

Storage instructions: -20°C. Do not aliquot the antibody

Recommended dilutions: WB: 1:2,000-5,000

Optimal dilutions should be determined by the end user.

Specificity: The His tag antibody can recognize C-terminal, internal, and N-terminal His-tag fusion proteins.

Form: Liquid

Reactivity: ALL


Western blot analysis of His recombinant protein with His Mouse mAb(6E8) HRP Conjugated diluted at

11:2,000

21:5,000 


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