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flag-HRP Mouse Monoclonal Antibody


The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.

Product Name: Flag Mouse Monoclonal Antibody-HRP conjugated

Isotype: IgG1

Clone Number: 3C6

Storage Buffer : PBS, pH 7.4, containing 0.02% kathon as Preservative and 50% Glycerol

Storage instructions: -20°C. Do not aliquot the antibody

Recommended dilutions: WB: 1:2,000-5,000

Optimal dilutions should be determined by the end user.

Specificity: The Flag tag antibody can recognize C-terminal, internal, and N-terminal Flag-tag fusion proteins.

Alternative Names: DDDDK epitope tag, DYKDDDDK epitope tag, ECS epitope tag

Form: Liquid

Reactivity: ALL




Western blot analysis of Recombinant protein with BE7003 diluted at

1) 1:2,000

2) 1:5,000 




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