BCL2 is an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. BCL2 suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. It regulates cell death by controlling the mitochondrial membrane permeability.
Product Name:Bcl-2 Mouse Monoclonal Antibody
Clone Number: 7B6
Isotype: IgG1
Storage Buffer :PBS, pH 7.4, containing 0.02% sodium azideas Preservative and 50% Glycerol
Storage instructions:-20°C. Do not aliquot the antibody
Recommended dilutions: WB: 1:1,000~2,000 IHC: 1:200IF: 1:100-200
Optimal dilutions should be determined by the end user.
Specificity: Bcl-2 antibody detects endogenous Bcl-2 proteins.
Molecular Weight:26KD
Form: Liquid
Reactivity:H, R, M
Western blot analysis of Hela with Bcl-2 mouse mAb(7B6) diluted at 1:1,000.
Western blot analysis of 3T3 with Bcl-2 mouse mAb(7B6) diluted at 1:1,000.
IHC staining of Human tonsil tissue paraffin-embedded with Bcl-2 mouse mAb(7B6)diluted at 1:200.
IF analysis of Hela with BE3108(Left) and DAPI (Right) diluted at 1:100.