Flag-Tag(HRP Conjugated) Mouse Monoclonal Antibody
Catalog NO.：BE2006
Applications ：WB, ELISA
Reactivity ：ALL
| 货号 | 规格 | 品牌 | 库存 | 价格 | 数量 | 操作 |
|---|---|---|---|---|---|---|
| BE2006-10 | 10μl/支 | EASYBIO |
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| BE2006-100 | 100μl/支 | EASYBIO |
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The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.
| Product Name: | Flag-Tag(HRP Conjugated) Mouse Monoclonal Antibody |
|---|---|
| Isotype: | IgG1 |
| Storage Buffer : | PBS, pH 7.4, containing 0.02% kathon as Preservative and 50% Glycerol |
| Storage instructions: | -20°C. Do not aliquot the antibody |
| Recommended dilutions: | WB: 1:5,000-10,000 ELISA: Use at an assay dependent dilution |
| Optimal dilutions should be determined by the end user. | |
| Specificity： | The Flag tag antibody can recognize C-terminal, internal, and N-terminal Flag-tag fusion proteins. |
| Alternative Names： | DDDDK epitope tag, DYKDDDDK epitope tag, ECS epitope tag |
| Form: | Liquid |
| Reactivity: | ALL |
